The Chocolate War Assignment #2 Essay Example | Topics and Well Written Essays - 250 words

The Chocolate War Assignment #2 - Essay Example Surprisingly, Jerry went against the norm of the school, by declining to accomplish the school assignment the way other students did. Those who refused to accomplish such assignments faced serious repercussions. Before Jerry joined the school, other students used to dance to the tunes of The Vigils, since they feared the violent consequences that would erupt. The students, as well as The Vigils and the administration are astonished the moment they realized that Jerry declined to accomplish the assignment. This triggered the long awaited dominant violent act to erupt. Jerry has undergone metamorphosis from being a hero to being an enemy. At first, his fellow students were amazed at his courage to go against The Vigils’ wishes. However, the moment the students realized that Jerry was going to face violent consequences; they abandoned him and went ahead and sold their chocolates. Furthermore, when Jerry was informed that he was to take part in the boxing face-off, his friend, Goober tried to stop him in vain â€Å"Sick. But I just heard about this and I had to come to stop you. For chrissake Jerry, don’t do it.† Jerry went ahead and answered â€Å"I think I can take him, Goob, I really do†¦Ã¢â‚¬  (Robinette 95). The violence finally exploded at the tail end of the play and Jerry faces serious injury and is taken to

Practical report Essay Example | Topics and Well Written Essays - 2000 words

Practical report - Essay Example The contents include fats, carbohydrates and proteins. The enzymes that break apart DNA are thereafter destroyed (Bruns 2007, 50). DNA content is then separated from other cell components. The researcher then precipitates the DNA and re-suspends it in a solution suitable for its studies. When extracting DNA from the cheek cells, saline solution used to rinse the mouth helps to prevent the cells extracted from splitting open or lysing too soon. Centrifugation separates the cheek cells from mouth wash used (Johannson 1972, 39). Spinning the mixture in a centrifuge settles the heavier cells to the bottom of the tube to form pellets. Saline solution pours away, leaving the clumped cheek cells at the bottom of the tube. Lysis buffer added to the cell clump splits open the cells to release DNA from inside the nucleus. The buffer contains soap that dissolves and breaks fatty membranes of the cells, buffer that maintains the pH of the solution and ions that increase osmotic pressure outside the cheek cell and aids in ripping open the cell membrane. Incubation in hot water helps denature cytoplasmic enzymes that break up DNA. Concentrated salt solution changes polarity of the solution under study. DNA elements dissolve in ionic solutions. This is as opposed to other components of the solution; proteins, carbohydrates and fats. ... The process is additionally useful in assessing and distinguishing the variable sizes of alleles. This discerning of allele sizes best takes place with the DNA strands placed at a single locus. Gel Electrophoresis also assesses the quantity and quality of DNA that is present in a sample (Komrakova 2006, 51). This method separates chemical molecules and compounds by charge and size. Substances that are separated are stationed in wells in the agarose gel and an electric field applied. Positively charged molecules and compounds move towards the negative terminal while the negatively charged particles and compounds move towards the positive anode. Larger and longer particles experience difficulty in moving across the mixture to the positive or negative terminal, and are suspended in the gel matrix. Smaller and shorter molecules move easily through the agarose gel matrix and take positions according to their polarity. When strained, the small sized segments form a tight band as they move at relatively the same speed. Type of medium and concentration of the gel determines the gel’s pore size and its ability to segregate same sized fragments. While polyacrylamide gels separate DNA segments differing by a base pair, agarose gels separate fragments of DNA differing by hundreds or more base pairs. Combs forming wells are placed into the gel as it solidifies and cools. The combs are then removed after the gel solidifies. Students can use gel electrophoresis in determining quality and quantity of the DNA matter they extract from their cheek cells. In day-to-day applications, the method is useful in fingerprinting or profiling, DNA sequencing and genetic